Multigroup analysis by gender?

Research model have three independent variable and one dependent variable. Basically i want to check the entrepreneurial intentions of business students. Nature of study is quantitative and i'll...

18 October 2020 1,176 3 View

Forcast of any Index is possible or not?

to forcast some indicator, can we directly forcast Index variable or we have to forcast all the poxies, used to calculate that index to forcast that index. for example to forcast inflation, can we...

29 November 2019 5,028 3 View

How to find rhamnose in plant (wheat) under stress and seed priming?

How to find rhamnose in plant (wheat) under stress and seed priming?

01 September 2019 5,478 2 View

To identify structural break, which method is appropriate?

to capture structural break in panel data series, whether we must add time dummy in regression equation or must apply chow test? which method would be better?

16 May 2019 157 3 View

How to perform a REFA (Regularized Exploratory Factor Analysis)?

Hello everyone! I am performing a personality study to unravel the personality dimensions in an African primate species. The group of subjects accounts for five individuals what makes my sample...

06 July 2014 839 5 View

How to perform a REFA (Regularized Exploratory Factor Analysis)?

Hello everyone! I am performing a personality study to unravel the personality dimensions in an African primate species. The group of subjects accounts for five individuals what makes my sample...

04 July 2014 4,101 4 View

Has anyone used DEA (Data Envelopment Analysis) in research work?

Could anyone suggest some useful and easy to understand references?

19 February 2014 5,487 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View