What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

20 November 2019 7,210 3 View

Can any one please suggest me that for micropore analysis of activated carbon which model gives relatively accurate value ?

I am new to micro-pore analysis. Recently, I have carried out low pressure isotherms (N2 @ 77 K and Ar @ 87 K) on activated carbon using a Quantachrome instrument. However, when I chose the...

21 April 2019 2,699 4 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Does anyone work with Diniobium Carbide (Nb2C)?

I would like to know if material is electrical conductive on nanosize domains. How different it is from NbC is Nb2C? Any literature?

19 June 2018 3,091 2 View

In your opinion, should ALL research articles be shared and accessed freely? Please give a reason for your answer (why)?

This will throw more light on the open access policy of publishing institutions

12 June 2018 7,439 3 View

Is it possible to create a part from an orphan mesh?

14 June 2016 1,343 3 View

Attached is an image of PPF stained with PTA. The PPF has nano cellulose particles. My question is this artifact from the stain?

Stained with PTA for 15 minutes. Bulk material is PPF with nano cellulose crystals.

22 May 2016 1,559 1 View

In the attached paper, the clue is Wernicke that guarantees thiamine deficiency. Was this sought as etiology?

Wernicke has occurred in hyperemesis with and without hyperalimentation and thiamine deficiency (TD) is the etiology of the hyperemesis. Since emotions are generated in the limbic system that is...

21 May 2015 6,476 1 View

No title

01 September 2014 7,367 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

How to perform the SEM, TEM characterizations to study the surface morphology of a Gold-coated PCF sensor?

I have designed an SPR based PCF sensor, which has a TiO2 and Gold coating. A reviewer has asked for the characterizations (e.g. SEM, TEM) to study the surface morphology. The reviewer has also...

06 December 2020 7,210 3 View

How we can isolate" distinguish" between Tyrophagus putrescentiae and T. entomophus ?

If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...

30 November 2020 9,087 3 View

Carbon dioxide sequestered as Blue Carbon in mangroves soil four times more than that of the green carbon, but obtained data does not reflect, why?

Mangroves have the ability to absorb up to four times more carbon dioxide by area than upland terrestrial forest ecosystems, carbon dioxide is stored as blue Carbon in the sediment of the...

27 October 2020 2,262 4 View

Can you help me explain these morphological changes in Human fibroblasts?

Hello I have these human fibroblast cells in culture for a while and they look fine with a typical fibroblasts morphology, At passage 8 the culture looks like a heterogenous culture with...

22 September 2020 7,856 3 View

What can we call this type of surface morphology formed in Cu2O at high laser power during the SLM process?

A kind of surface roughness is produced by the application of laser energy density at high laser power (> 140W) which looks like an open porous structure. This kind of structure is not observed...

18 August 2020 9,835 4 View

How may untypical structures inside the spores of arbuscular mycorrhizal fungi be identified?

I can not describe morphology of some AMF spores isolated from soil sample. Permanent slides in PVLG+Melzer, x600, light microscopy and phase-contrast microscopy. Pink stained spherical structures...

01 August 2020 3,498 3 View

Inflection POS-suffix dataset ?

Hello, I am trying to find a dataset of canonical suffix forms for Parts of speech tags. For example, ed->VBD (past tense), ing-> VBG. Is there any available dataset like this? Thanks!

01 August 2020 5,066 2 View