Hello,
We're tediously isolating PBMC to obtain primary macrophages for research. Why people don't establish an immortalised one like what has been done in mouse to get the immortalised bone marrow-derived macrophage (iBMDM)?
Dear Renfei!
Please You look at the article:
Chapter Immortalization of Murine Bone Marrow-Derived Macrophages
Thanks Alexandr Alexandr Chernov
I think I can refer to this protocol. Hope it works for human tissue as well.
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