Is there any possibility to convert plaque forming units per mL (p.f.u/mL) to nano grams per ml (ng/mL)?

More Samira Hosseini's questions See All

How to report if someone uses my profile picture?

I have found a researchgate account with one single publication which is not my publication, but interestingly the author uses my profile picture! how should I solve this problem? There is no way...

01 February 2016 6,255 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Nano Orange protein quant on the Quantus fluorometer?

Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...

05 August 2024 8,946 3 View

Dengue in Southern states in India?

There is rapid surge of dengue cases in Southern parts of India. However there are no cases till now in the setting I work in Northern India. Besides dengue surveillance and local preventive...

14 July 2024 2,935 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,482 3 View

Unexpected 35 kDa Band Observed During Expression and Purification of Dengue 3 NS5 RdRp Domain ?

Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...

13 July 2024 9,950 3 View

Intracerebroventricular (ICV) AAV inj.?

I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...

12 July 2024 781 0 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Does anyone know how to improve calcium-imaging fluorescence with GRIN lenses?

Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...

08 July 2024 5,178 2 View

Why do I have two different bands from the same virus after PCR?

I get two different bands from BCMV after PCR

07 July 2024 2,112 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View