Is there any possibilities for bacteria contaminants in medicinal plants produce toxins after the exposure to gamma radiation? - FAQS.TIPS

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Nanogram (ng) of DNA is equal to how many microliters (ul)?

Hi everyone. I have some confusions on ng of DNA and microliters (ul). I've read several PCR protocols which mentioned on the ideal concentration of DNA template per reaction. I couldn't...

02 March 2019 8,907 5 View

What is actually happen when I got band detected on gel electrophoresis at 10,000 bp?

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...

11 December 2018 9,557 4 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 2,241 1 View

Hi all. I have a question on statistical analysis. How we want to calculate the differences between storage periods with bacterial counts?

Basically I want to look for the differences between storage periods and bacterial counts. However, I don't know which tests that I can use to determine the significant differences. For example,...

11 December 2018 8,956 4 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 4,841 1 View

What is actually happen when I got band detected on gel electrophoresis at 10,000 bp?

I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...

11 December 2018 5,812 1 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 6,855 1 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 4,749 1 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 5,188 0 View

What is exactly happened when I got DNA band on gel electrophoresis and it is detected at 10,000 bp marker?

I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...

11 December 2018 7,276 3 View

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I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

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Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

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Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

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What is this fern?

Please help me to identify this fern species. Thank you in advance.

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Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

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What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

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How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

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