You can please refer to the links below to obtain certain valuable informations:-

1. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2737441/

2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC497632/

3. http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2010.01939.x/full

4. http://www.sciencedirect.com/science/article/pii/S094450130600036X

5. http://aem.asm.org/content/67/7/3195.full

6. https://www.researchgate.net/publication/7876719_Rapid_identification_of_Streptococcus_pyogenes_with_PCR_primers_from_a_putative_transcriptional_regulator_gene

Article Rapid identification of Streptococcus pyogenes with PCR prim...

One of above mentioned papers:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC497632/

Use of tuf Sequences for Genus-Specific PCR Detection and Phylogenetic Analysis of 28 Streptococcal Species

A 761-bp portion of the tuf gene (encoding the elongation factor Tu) from 28 clinically relevant streptococcal species was obtained by sequencing amplicons generated using broad-range PCR primers. These tuf sequences were used to select Streptococcus-specific PCR primers and to perform phylogenetic analysis. The specificity of the PCR assay was verified using 102 different bacterial species, including the 28 streptococcal species.  There was cross-amplification with DNAs from Enterococcus durans and Lactococcus lactis. However, the 15 to 31% nucleotide sequence divergence in the 761-bp tuf portion of these two species compared to any streptococcal tuf sequence provides ample sequence divergence to allow the development of internal probes specific to streptococci.

The Streptococcus-specific assay was highly sensitive for all 28 streptococcal species tested (i.e., detection limit of 1 to 10 genome copies per PCR).

Actually i plan to used a pair of primer for screening only streptococcus.

Thank you very much. I found what i was looking for after get answer from both of your. 

Regards

Hey rortana, I am in the same situation as you. What primers did you find and where?

Hi I am looking for primers to identify strep species. I tried using tuf gene it works well but we got amplification for E.fecalis too. Still looking for Strep species specific primers. Any leads is highly appreciable. Thank you.