The creation mixture comprised 0.1 ml of the enzyme extract, 50 mM Tris-HCl pH8.5, and 10 mM L-phenylalanine. The enzymatic reaction was incubated at 40 °C, while a control reaction was performed in the same conditions but using D-phenylalanine as substrate. The cinnamic acid formation was followed by absorbance readings at 30 min intervals in quartz cuvettes at 290 nm wavelength for 2 hours.