Abubakar Abdullahi Lema

6 Questions 9 Answers 0 Followers

Questions related from Abubakar Abdullahi Lema

I have purified anthocyanin, i used cyanidin -3-glucoside as standard?

so i got the peak for cyanidin glucoside correspond to the reference standard but i have other peaks in my samples how can i identify them?

05 March 2022 3,627 7 View

Hello colleagues. Please is chalcone synthase CHS gene conserved in all plant and can I use CHS primer for rice in Roselle ?

Please is chalcone synthase CHS gene conserved in all plant and can I use CHS primer for rice in Roselle or new one has to be designed ?

16 November 2021 3,901 0 View

I have calculated Total Anthocyanin Content (TAC) using the following formula. must i run the standard curve or this will be enough ?

Absorbance (A) = (Aλ vis-max x A700) pH 1.0 0 (Aλ vis-max x A700) pH 4.5 TAC (mg/L) = (A × MW × DF × 1000)/(ε × l) MW: the molecular weight, calculated as cyanidin-3-glucoside (449.2); DF: the...

14 October 2021 4,179 3 View

Is there any other substrate other than D-phenylalanine for determination of phenylalanine ammonia lyase?

The creation mixture comprised 0.1 ml of the enzyme extract, 50 mM Tris-HCl pH8.5, and 10 mM L-phenylalanine. The enzymatic reaction was incubated at 40 °C, while a control reaction was performed...

05 October 2021 3,745 1 View

How to prepare 20, 40, 60, 80, and 100micro molar hydrogen peroxide?

Prevention of Cellular Oxidative Damage by an Aqueous Extrac...

05 October 2021 3,083 3 View

Hello. Please i want to know how to prepare standard (cyanidin-3- glucoside) for determining total anthocyanin using pH differential method ?

15 September 2021 3,818 5 View