Hi, recent days I try to follow this protocol to set up MERFISH. Chapter RNA Imaging with Multiplexed Error-Robust Fluorescence In Si...
However, I have some problem to download a program called OligoArray - which design multiple probe targeting specific transcripts and score it.
I also check some other probe design sites or program, but non of them satisfied my purpose - [design 30nt (quite long) X 100 different probes that targeting single mRNA X for 130 genes].
So, is there any other program/sites for those kind of probe design?
Thank you for reading.