07 July 2014 1 2K Report

I want to induce cancer in c.elegans using some chemical. so can somebody please tell me the chemicals which can be used for this purpose? Is it possible with benzopyrene, DMBA, diethylnitrosamine or any kind of radiation?

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Do all the mRNAs of pox virus are polyadenylated at 3' end?

Hello everyone, In eukaryotic cells, all the mRNAS are polyadenylated at 3' end. I want to ask whether this is the same case for pox viruses also. Do all their mRNAs are polyadenylated at 3'end...

04 May 2019 3,309 2 View

Can we use freezed nuclear lysate forDNMT activity analysis from cells?

Hello everyone. I want to do DNMT activity analysis by using prostate cancer cells. I want to know whether we can store the nuclear lysate for 2-3 days in -20 degree before performing activity...

05 June 2018 6,568 7 View

Do we need to quantify bisulfite treated DNA for using it as a template for methylation specific PCR?

Hello everyone I have bisulphite converted my DNA using kit from Zymo research now I have to do methylation specific PCR. I have to check effect of my drug on methylation status of diff. genes...

10 November 2017 8,380 4 View

Can we use autoclaved DEPC milliQ water for bisulpgite conversion and methylation specific pcr?

I am going to do ms pcr but I have doubt on water milliQ water because previously RT pcr result was not coming by using that water. So I borrowed water from another department but that water was...

09 October 2017 4,011 4 View

RBC contamination in WBC pellet during DNA isolation from blood?

Hello everyone, I isolated DNA from freshly taken Human blood but during processing I was getting RBC contamination in pellet .I washed with RBC lysis buffer 6-7 times followed by...

09 October 2017 5,451 3 View

Can we use 3-4 month old DNA for methylation studies?

Hello, I have 3- 4 month old DNA stored at  - 2O degree. Can I use thisDna for bisulphite treatment followed by methylation specific pcr? Thank you

31 December 2016 5,366 5 View

DNMT activity assay?

Hello, I want to do  radioactivity based DNMT activity assay. Can somebody upload the whole prtocol for this?  Thank you

06 July 2016 2,258 5 View

Has anyone done RT PCR for Cyclin D2 , p21 and p27 in DU145 cells ?

I have done RT -PCR for Cyclin D2, p21 and p27 in du145 , but did not get any band . in p21 , there was v. faint band but when I repeated the same , I did not get any band for p27. does these 3...

03 April 2016 928 1 View

How to freeze LNCaP cells?

What is the best way to freeze LNCaP cells? ATCC recommends complete medium plus 5% DMSO. some people use 10% DMSO, 20% FBS and rest complete medium. Some use 90% FBS and 10% DMSO. Please tell me...

31 December 2015 2,459 3 View

Why do we need to do the conditioning of vessels with media or PBs in rotary cell culture system?

we purchased RCCS from synthecon Inc. In users guide it is mentioned to prepare vessels by  rinsing with media or PBS for 5 mins to overnight. I want to know the purpose of doing this...

31 December 2015 4,483 2 View

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