Even I tried different rate of lights , low level of auxin (till 0 level) and high level of different cytokinines , still jatropha explant produced a much more of callus.
Is there any one anothere solution for this problem ?
You may go through the attached file, probably you may get the answers.
Just curious, is this project to do with using Jatropha for biofuel production?
I push Brachypodium from callus to shoot production using the John Vogel lab protocol for regeneration (below). You could give this a try?
Regeneration media (RM): per L, add 4.43 g Linsmaier and
Skoog (LS) basal medium and 30 g maltose. Adjust to pH 5.8
with 0.1N KOH, and then add 1.0 ml of 0.2 mg/ml kinetin
stock solution. Add 2.5 g phytagel for hygromycin or BASTA
selection or 5 g phyto agar for paromomycin selection. To prevent
clumping, ensure bottles are dry before adding phytagel
or agar. Autoclave media on a liquid cycle for 45 min. After
autoclaving, cool media to 65 °C in a water bath. When media
has cooled sufficiently, transfer media to a sterile hood. (Add appropriate antibiotic/herbicide for selection of transgenic callus), and pour media into
petri dishes.
Incubate plates in the light (cool-white fluorescent lighting at
a level of 65 μEm/m 2 /s with a 16 h light/8 h dark cycle) at
28 °C. Callus will start to turn green and shoots should appear
in 2–4 weeks.
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