Is there any method to deglycosylate a protein (N and /or O gylcosylations) without denaturation the protein itself?

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Sven M. Bergmann

Thanks a lot Katja, that's it!

Best wishes

Sven

Joanna Kruszewska

This publication about enzymatic deglycosylation could help.

http://www.ncbi.nlm.nih.gov/pubmed/24182931

Look also at the BioLabs manual to their special kit “Enzymatic removal of N- and O-glycans using PNGase F or the Protein Deglycosylation Mix”.

https://www.neb.com/~/media/NebUs/Files/Application%20Notes/appNoteP0704.pdf