Tim Tolentino

3 Questions 29 Answers 0 Followers

Questions related from Tim Tolentino

Could someone please tell me the advantage of having a cell line that is an NLK knockout?

I've read claims that a cell line with an NLK Knockout is "great for drug discovery." I realize I need to search the literature and read what is out there, but I'm short on time and overworked at...

07 July 2019 8,266 1 View

If you were to choose only three cell lines from the NCI-60 panel, to use to test anti-cancer drugs, which three cell lines would you choose?

I have been testing compounds for for their anti-cancer properties. The National Cancer Institute has 60 cell lines they test potential anti-cancer drugs on. If funding, and time, and lab...

07 July 2019 2,815 5 View

How efficient is whole plasmid PCR?

I am considering having students amplify whole plasmids through PCR. I've never tried 'whole plasmid pcr' and so have no experience with it. I'm wondering if it is efficient or practical.

09 September 2013 1,179 4 View