I have done IF for GFAP focusing on hippocampus (CA1, CA2, CA3 and DG). I need to do quantification for GFAP positive cells. Is there any valid method for these?
Hopefully, I understood your question correctly. If you obtain some images of slices 9as more as possible), you could use any macros of program ImageJ dependently of the aim of your experiment: quantity of GFAP positive cells or GFAP positive cells covered area.