How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

My question is that how can we select the unique peptides ?

Hi all Scientists; I have similar question. Can you please help me in selecting unique/distinct peptides in case of LC-MS/MS analysis? I have done salivary gland proteomes for...

26 January 2021 7,488 3 View

How to design reverse primer with ambiguous nucleotides?

How to design, reverse primer with ambiguous nucleotides?

03 January 2021 6,762 4 View

Why does the DNA's elution decrease after centrifuging with spin column?

02 January 2021 987 4 View

How to synthesize pyrrolium ionic salt?

I am keenly interested in the synthesis of heteroaromatic ionic salts for use as an organic template. I want to synthesize alkyl pyrrolium chloride or bromide through a simple addition reaction...

30 December 2020 2,778 3 View

How can large volume ( 100 liters or more ) of carbon dots solution be purified and sieved cost-effectively?

Articles describing the use of carbon dots in agriculture have been increasing recently. Again, studies have found that carbon dots size and shape and some other features are effective. Therefore,...

30 December 2020 1,139 3 View

How to Modeling and simulation for launch vehicle?

Modeling and simulation for launch vehicle Title of graduation project for a master's thesis in mechanical engineering, specializing in Control Search assistance required? Thank you

28 December 2020 9,000 1 View

How to calculate average habitat suitability change between 2 different SDM/ENM?

Hi guys, I would love to know how I could go about solving the above question. I currently have 2 different species distribution models, one created with the current climate projection and the...

14 December 2020 864 8 View

What are good M.Sc thesis topic suggestions in construction management (specially BIM & Waste management area)?

My prefered area is in Waste management & BIM , where I have read about using 4D BIM & 5D in waste management , but I have not reached a significant research gap to work on it, so is...

03 December 2020 1,294 2 View

RBC's contamination during DNA extraction ?

Hi there! currently i am working with spin column kit for DNA extraction I've put almost 600 uL of buffy coat. after a lot of washing, the RBC's remained stucking in filter? How could i get rid...

25 October 2020 8,712 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View