Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

HT22 purchase in Greece?

Hello everybody, I am looking for a specific cell line HT22 but I haven't found any company that could import it in Greece. Has anyone faced that problem before? Is there any alternative way that...

01 April 2019 1,917 3 View

Hello, Can anyone help me use krona tool for visualising my NGS data?

My files contain data in percentages or in counts.Does Krona tool need bioinformatic language to be used? Thanks in advance.

27 January 2019 1,758 2 View

How can we estimate how much is the sensitivity of a Chi Squared test to the number of classes?

Should we run the experiment with different number of classes and see if there is any difference?

23 August 2018 3,780 2 View

Can I use a 2nd generation pTet-ON with a 1st generation TRE (for Tet-ON inducible expression) ?

Hello all! I would like to integrate a pTet-ON plasmid in the genome of a stable cell line, which already contains a TRE (Tet Response Element). I was thus wondering whether I could use a 2nd...

22 February 2018 2,437 1 View

Why do I need to wash the entanol from ethanol stored samples with Tris-HCl before extracting DNA?

I worked at a laboratory and before extracting DNA, I washed of the ethanol with Tris-HCl, in order to get rid of all the ethanol before starting the extraction process, but I did not understand...

02 February 2018 9,725 1 View

Yeast fermentation medium recipe??

Can anyone please explain what is a fermentaion medium and a yeast suspension and how to make them? I have attached the paper that these two are mentioned and I have highlighted them in the...

03 January 2018 3,151 2 View

How can i dilute carvacrol (oil) ?

I have read in some papers that carvacrol can be diluted in Tween 80 (1%) and distilled water. I have tried this but carvacrol wasnt fully dissolved. After that i tried to dissolve carvacrol in...

09 August 2016 6,070 7 View

How I can get the Organizational Commitment Questionnaire?

Dear Dr. Meyer, I would like to inform you that I am a student candidate for a PhD in Cyprus University of Technology and my subject relates to job satisfaction, leadership style and commitment of...

27 March 2016 6,828 15 View

Is a standard curve required for each assay when we use an elisa kit?

Does anybody know if i can use some wells ( about 10 wells)of the TNF-a elisa kit in order to try my samples? I have not used a kit before and i wonder whether i can use some wells without running...

25 March 2016 5,274 17 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View