I am detecting mouse proteins in my primograft samples. I am looking for human specific loading control, I have tried GAPDH, Tubulin, Clathrin but they all detect both human and mouse proteins. There are some loading control antibodies claimed to be human specific but when I blast their immunogen sequence I found them cross reacting with mice and other species.
Hasan: Cross-react or not relies on the sequence(s) of even the same protein from different species. So, you can look for such Abs that only react to mouse sequence but not human one. In my experience with our own study, we found Abs from a commercial source. Good luck.
Don't know about mouse sequence homology, but Origene has anti-TBP loading control derived from relatively small immunogenic peptide of human TBP: DQNNSLPPYAQ-C
Our listing of their Ab is here: https://www.linscottsdirectory.com/antibodies/origene-technologies/TBP-12940867
Please contact them directly for more details.
Hasan:
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
References
1. Williams, B.P. et al. (1988) Immunogenetics 27:322.
2. Chen, Y. et al. (2003) Cell Res. 13:251.
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