Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Which software is best for geochemical data analysis and graphics for Geochemical Modelling of Igneous Processes?

I'm familiar with Geochemical data analysis software such as GCDKit, PetroGraph, and Petroplot etc. which one is best and suitable for doing Geochemical Modelling of Igneous Processes? I need...

22 December 2020 9,300 2 View

Does anyone know of an anti-rat CLEC-2 antibody for IHC?

I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...

06 December 2020 3,511 0 View

Help finding right expression to fit data?

Hello, I have some data that looks like the line on the right, but I'm not sure what function to use. It seems like an exponential added to a line, or something of the like. I think it may be the...

18 November 2020 2,357 3 View

Single-turnover kinetics question?

It is my understanding that single-turnover reactions isolate the chemical step, as opposed to being comprised of the chemical step + product dissociation as in kcat. Here's my issue though....

13 November 2020 2,494 7 View

How to make a high spatial resolution land use land cover map in a 10 year temporal resolution with respect to Indian scenario?

I want to create a large scale land use land cover change map of a village of India in the 10-year interval. Which one is the appropriate satellite image for this. and anybody kindly explains the...

12 October 2020 3,996 6 View

Contrasting singular means?

Does anyone know of a method or approach in SPSS to contrast singular mean scores of overall samples, based only on the overall mean value, standard deviation figure and sample size? The data is...

29 September 2020 5,872 6 View

How to calculate new human dose based on In-vivo animal study results?

I have performed In-vivo study in rate model for new formulation as well as marketed formulation. Based on results new formulation have significant improvement in all PK parameters. Oral dose...

20 August 2020 2,209 1 View

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09 July 2020 9,914 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View