We are looking for a good antibody for plasma/serum zonulin (haptoglobin 2). There are few antibodies in the market but the problem most of them don´t have any citation. Some of them are not correctly mapped. We want to see all of the possible bands (alpha chains, or intact peptide seq). We are planning to do western blot.
Thanks!
zonulin is PRE-haptoglobin--zonulin was discovered by Dr. Alessio Fasano who has also developed an inhibitor for potential therapeutic use---it is not an antibody but if such is available would think Dr Fasano now at Mass. Gen. in Boston would know
According to Alessio Fasano (2002), Zonulin modulates the permeability of tight junctions between cells of the wall of the digestive tract. Therefore, Zonulin is not an antibody but a protein where it is synthesized mostly in the intestinal barrier and liver.
YES! we know Zonulin is not an antibody BUT the question was --"are there any good antibodies" to Zonulin---I am sure that Dr Fasano the discoverer of Zonulin would know what are the best and hopefully the seeker of that answer has gotten his answer by now!!!
Quite old question, but it may still be interesting to someone else. Unfortunately, there are only predatory ELISA kits commercially available (for instance Immundiagnostik and Cusabio as the most common, but there are many others) for "zonulin", to understanding of zonulin being the same protein as pre-haptoglobin 2 has to be carefully taken, as a quick BLAST search will give that sequence to be gamma immunoglobulin heavy chain.
This considered, one should look at these papers on Immunodiagnostik kit ( Article Widely Used Commercial ELISA Does Not Detect Precursor of Ha...
)and on CusaBio kit (Article Serum zonulin as a marker of intestinal mucosal barrier func...
)Zonulin as shown in the original publication is definitely not pre-haptoglobin 2. However, this paper ( Article Identification of human zonulin, a physiological modulator o...
) suggests that it is, even if the sequence of zonulin has still not been described whatsoever. There is a good pre-haptoglobin 2 antibody, but it is not commercially available (Article Development of monoclonal antibodies to pre-haptoglobin 2 an...
), but this one fits genotyping of Hp genes. Have fun!
@Anthony: Thanks for your suggestion! We did get free antibody from Dr. Fasano
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