Hi....I am working on ipomoviruses infecting vegetables in Iraq and I wonder if there is any primer set specific for detection of the genus Ipomovirus? As the only available option for the genus detection is Potyvirididae specific primer set.
Hi Nawres, You will have to search and there may be. The relevant papers are:
Gibbs, A. and Mackenzie, A. (1997) A primer pair for amplifying part of the genome of all 'potyvirids' by RT-PCR. J. Virol. Meths 63, 9-16.
Gibbs, A.J., Mackenzie, A.M. and Gibbs, M.J. (2003) The ‘potyvirid primers’ will probably provide phylogenetically informative DNA fragments from all species of Potyviridae. J. Virol. Meths. 112, 41-44
Zheng L, Wayper PJ, Gibbs AJ, Fourment M, Rodoni BC, Gibbs, MJ (2008) Accumulating Variation at Conserved Sites in Potyvirus Genomes Is Driven by Species Discovery and Affects Degenerate Primer Design. PLoS ONE 3(2): e1586. doi:10.1371/journal.pone.0001586.
The region of the ipomo genome for these primers is intact, and I will attach some 'screen grabs' from a quick alignment I made this morning - nts and AA sequences, names and 100% conserved sites.
If you are uncertain, please email again.
Best wishes Adrian
Dear Prof. Gibbs, I really appreciate your time and efforts dedicated to get me the answer, your data is so valuable , I am going to test the potivirid primers to detect ipomoviruses....
Many thanks
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