Hossein,

bacteriophage genomes are very diverse, but you can design primers for some families, following  applied approaches:

Edelman DC, Barletta J. Real-time PCR provides improved detection and titer determination of bacteriophage. Biotechniques. 2003 Aug;35(2):368-75. PubMed PMID: 12951778.

Matveeva OV, Foley BT, Nemtsov VA, Gesteland RF, Matsufuji S, Atkins JF, Ogurtsov AY, Shabalina SA. Identification of regions in multiple sequence alignments thermodynamically suitable for targeting by consensus oligonucleotides: application to HIV genome. BMC Bioinformatics. 2004 Apr 29;5:44.

Yong SF, Ngeow YF, Tong YK, Ong JT. Real-time PCR detection of male-specific

coliphages. Malays J Pathol. 2006 Dec;28(2):79-82

Alex

Hossein,

bacteriophage genomes are very diverse, but you can design primers for some families, following  applied approaches:

Edelman DC, Barletta J. Real-time PCR provides improved detection and titer determination of bacteriophage. Biotechniques. 2003 Aug;35(2):368-75. PubMed PMID: 12951778.

Matveeva OV, Foley BT, Nemtsov VA, Gesteland RF, Matsufuji S, Atkins JF, Ogurtsov AY, Shabalina SA. Identification of regions in multiple sequence alignments thermodynamically suitable for targeting by consensus oligonucleotides: application to HIV genome. BMC Bioinformatics. 2004 Apr 29;5:44.

Yong SF, Ngeow YF, Tong YK, Ong JT. Real-time PCR detection of male-specific

coliphages. Malays J Pathol. 2006 Dec;28(2):79-82

Alex

My understanding is that the morphology of the virion (using TEM) was the only way to identify the family. We have tried to design primers to identify clusters just within mycobacteriophage, and the genomes can be so diverse that even doing that is difficult, depending on which cluster you are examining. I don't know if there is enough of DNA conservation in one of the head or tail gnes across all bacteriophage in a family to use that gene. But I would be very skeptical that this is even possible, depending on the host you are using.

I am working on Acenitobacter pahges, so there is restricted numbers of phages for every bacterium? I will take a TEM picture and probably it will help me to identify its family, but for its genome sequencing is I don't have any clue for primer