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sorry I dont know
I used I-TASSER to predict the 3D structure of my protein of interest and PyMOL with APBS plugin to add a surface showing the electrostatic potential. Then, with PyTMs plugin, I added three...
25 January 2024 6,228 0 View
I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry. Thank you in advance.
01 January 2024 6,335 1 View
I am using PyMol with PyTMs plugin to visualize possible phosphorylation sites on a protein. I am wondering how I can use the program to calculate and show an optimized protein structure to avoid...
26 November 2023 7,663 1 View
I need to anneal mm-scale samples made on Si substrates up to 600°C. To temporarily mount them, I've seen things like high temperature epoxies, tapes, and pastes as well as mechanical mounting...
10 November 2023 4,327 3 View
Hello! I have used Promega RealTime-Glo Annexin V Apoptosis Assay but the results looks very weird and I would like to know if someone else came across that kind of trouble. The highest...
15 October 2023 4,813 0 View
I am doing immunofluorescence on tissue sections. After the deparaffinization I do the antigen retrieval, but I think is destroying some nucleolus structures I am trying to label. Any...
22 August 2023 4,503 2 View
I am looking for a measure/scale suitable for assessment in classrooms to measure cultural competences (cultural sensitivity and self-awareness)? I will be grateful for any advice :) Best...
02 January 2023 1,268 2 View
Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...
26 November 2020 5,242 4 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
21 July 2020 2,043 4 View
Im planning to do the carburizing of my steel sample, but I want to prevent one part of it by copper plating and I was wandering if there is some indication about the copper coating created to be...
08 February 2020 4,958 0 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.
23 June 2024 2,287 7 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...
13 June 2024 9,936 5 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View
What is the difference between absorption and adsorption?
30 May 2024 4,447 27 View
I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...
28 May 2024 4,907 0 View
