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sorry I dont know
Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...
26 November 2020 5,080 4 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
21 July 2020 1,846 4 View
I understand that the compressed air for the laser optics purge (first step of cleaning) such as mirrors or lenses should be "clean", but does anybody can explain me what really does it mean...
01 April 2019 8,021 5 View
I would like to buy graphite target for further DLC films creation by PLD technique, does anybody have some good experience with company offering it in terms of reliability and good...
14 March 2019 8,314 3 View
Do you know where can I buy DiFi cell line? There are not available in ATCC, ECACC or DSMZ.
20 July 2017 3,772 1 View
Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...
19 June 2016 2,867 3 View
What do you think about using 8-channel head array coil?
04 January 2014 8,779 2 View
Dear fellows, I am currently working around specified subject. As part of my investigation I recruit volunteers, able to provide faecal tests results, suitable for ASD patients and control....
01 January 1970 6,630 3 View
I'm analysing the relation between the work with literary drama on the stage in the theatre and the theory of new philology (medieval texts). Do you have an example for it in the contemporary...
01 January 1970 8,329 2 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...
17 February 2021 4,844 4 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
16 February 2021 2,377 7 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
09 February 2021 5,155 5 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...
01 February 2021 780 3 View
I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...
17 January 2021 5,099 4 View
Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...
14 January 2021 4,383 8 View
Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...
05 January 2021 230 3 View
