How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

Are there commercially available fluorescence-tagged Influenza A virus?

30 November 2020 9,445 1 View

What tests for: 1. differences between continuous variables when grouped with different categorical ones; 2. significance of potential relationships?

Hello. I was wondering if anyone had suggestions in terms of what exploratory analysis to carry out for my dissertation with the following data. I measured N=26 participants pre and post an...

09 August 2020 5,256 5 View

Wavy looking bands In western blots?

Hello everybody, from some time, I keep having waving bands in the lower part of my gel, like the proteins are not running on a flat front. I cast my own gels and run them at 90V at the beginning...

26 May 2020 937 3 View

SURVEY - how people changed attitude toward green spaces & urban forests during the COVID19 quarantine?

https://vub.fra1.qualtrics.com/jfe/form/SV_5bSsVfI1g1VgI7j While shaking our society and turning our way of living upside down, the COVID19 pandemic is also putting the relation between nature...

15 May 2020 2,339 8 View

Direct Input of the Occupation Matrix?

I'm implementing the Occupation Matrix Control scheme (OMC) in VASP DFT+U calculations via direcct input of the Occupation Matrix. This requires an external input file (OCCMATRIX) containing the...

17 March 2020 1,921 1 View

Glycerol topology/structure for GROMOS87/Berger forcefield?

Hello all, I need the topology /structure file of the glycerol for the GROMOS87 forcefield. Is some repository available online or do someone know how can I derive them by myself (maybe some tool...

12 March 2020 8,858 2 View

How to interpret moderating effect in PLS?

I am running a Partial Least Squadre Path Modelling in R and I have a Moderating effect (explained by one scalar variable) between my exogenous x and my endogenous y. The only variable that...

25 February 2020 8,022 4 View

Recombinant protein for antibody neutralization?

I need to understand if an antibody I'm using is specific or not. The antibody is not validated. In immunoblotting, the antibody detects a prominent band at the right molecular weight and two...

29 April 2019 4,752 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View