i am following two assays for antioxidant activity one is hydrogen peroxide and ammonium molybdate but am getting negative absorbance in control or in test sample what i suppose to do now?
Hello,
Say what protocol you used, step by step. Also, I dont hear about antioxidant assay using hydrgen peroxide and ammonoum molybdate, but catalase asay.
Sir its hydrogen peroxide scavanging assy by ruch el al and total antioxidant capacity (phosphomolybdenum method) by prieto et al
Did you measured the absorbance of hydrogen peroxide at 230 nm in a UV Quartz Cuvette ??? did you prepared a "blank" solution containing the phosphate buffer without hydrogen peroxide?? Check the spectra of your test sample on the same buffer. Maybe your sample is having fluorescence.
Yes sir have used phosphate buffer without hydrogen peroxide as a blank and am using quartz cuvette only
Dear Vipin Nagda
what is the control did you use in this case?
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