Rdf of atoms from a COM or point in lammps or VMD?

Hello all, as I understand there isn't any straight way to get an rdf of atoms from the center of mass or a coordinate of specific atoms in lammps. I wonder if anyone know any trick or way to get...

16 February 2021 2,517 2 View

Could anyone help me about my problem with getting current in armchair MOS2 nanoribbon in transiesta?

Dear siesta users I am using transiesta code to get the I-V characteristics of Armchair MoS2 nanoribbon as scattering (with 8 unit cells) and electrode material. All the calculations (electrode,...

12 October 2020 4,306 6 View

Performance evaluation of a Crowdsourcing platform?

In order to evaluate the performance of a Crowdsourcing platform from open innovation point of view, what indicators should be considered?

20 August 2020 8,424 6 View

About use of Fluoranthene as standard in HPLC?

Dear all; I want to work on the ecotoxicological effect of PAH (fluoranthene) on different organism species.... But I can not find its analytical standard in my country, Can I use Flournthene 98%...

05 July 2020 2,736 7 View

What kind of dialysis cassette is needed for CaCl2 in silk fibroin dissolving?

Hi! Recently, I am working on silk fibroin extraction for biomedical application. I have some problem in details of extraction and dissolving of silk fibroin. For example, I don't know what kind...

26 June 2020 3,927 1 View

What kind of dialyses cassette is require for extraction of silk fibroin base on nature protocol 2011 (Attached in following)?

I have some question about the laboratory process of extraction silk fibroin from Bombyx mori base the Prof. Kaplan protocol. How much Silk cocoon need for 20 experiments? How dialyzes the final...

15 June 2020 8,694 1 View

No title

I'm trying to collect milk from our knockout mice (at the end of the lactation stage) to isolate milk exosomes. But I have a hard time with milk collection from mice. I have used either pump or...

22 January 2020 4,527 1 View

How to design primers for gene variant or isoforms? Preferably by Allel ID?

I am trying to design some priers for RT-PCR. As my genes have many isoforms, so I have to design primer for conserved sequences. but the results aren't satisfactory.

17 March 2019 3,346 3 View

Why is it used in bioassays studies with essential oils for effect pesticidal from acetone solvent instead of DMSO?

In bioassay studies, the effect of essential oils is used as an insecticide from acetone, but is used to study the microbial activity of essential oils from DMSO solvents. Why?

12 March 2019 5,664 7 View

Which type of surface modification should be used to match metal and graphene?

graphene is the reinforcement in the metal matrix

26 February 2019 4,160 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View