Dear Mauro,

The only difference I see is that in aerated cultures filaments are better dispersed and light uptake is maximized. Also, as more CO2 is "injected" in the cultures, it can favors growth. However, another effect I have noticed is in the length of the filaments, which is usually shorter in aerated cultures. My hypothesis is that bubles brake the filaments into shorter ones.

Best regards,

Aloysio.

Thank you, Aloysio.

I've seen some of these results (mainly about the filaments lenght), too. But, in other hand the CO2 input by aeration can cause a decrease in the pH (formation of H2CO3), in non buffered medium, which results in a greater acclimatation phase in C. raciborskii. I'd like to know if someone else experienced these results. Maybe the kind of inorganic carbon available could limit the growing... 

Do you aerate all your cultures? 

Thank you again for your attention and kindness!

Best regards,

Mauro 

Dear Mauro,

From my observation: aeration of Microcystis aeruginosa culture favors growth, while for Anabaena flos -aquae biomass accumulation I observed decrease in comparison to control (5-10 % in 30 day of cultivation). I suppose that for cyanobacteria which naturally preferring the top of the cultivation media (for example Anabaena) shaking them once or two times for day should suffice.

Best regards,

Ariel

Thank you for replying me, dear Ariel.

Best regards,

Mauro 

Dear Maruro, my experience in Nostoc spp. giving aeration could faster the growth of culture. I also experienced that the filament of Nostoc became shorter. I don't aerate my stock culture, just shaking them manually.

regards,

dian

Thank you for replying me, dear Dian. 

Did your strain reach a great biomass? 

Best regards,

Mauro 

aerated grow faster, non-aerated grow slower

Actually, yes. For culture above 2L aeration is important. Wish u luck with yours :)

Nice, Dian. Thank you! 

Dear Farrah, I'd like to know if anyone had an experience where 'Cylindrospermopsis raciborskii' didn't grow in aerated condition. Does is great growth in an aerated condition a pattern? I'm investigating which abiotic conditions associated to aeration drives a better growth in cyanobacterial cultures.