How to test the equality of two proportions with multiple samplings?

02 February 2021 9,574 5 View

How to compute pressure drop on liquid ammonia line?

I have to analyze an existing ammonia line to estimate the pressure drop in it. The line is designed to transport liquid ammonia. I can compute the pressure drop with standard friction factor...

21 November 2020 5,494 3 View

Problem executing MrBayes. Help?

Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...

18 November 2020 3,867 3 View

How can you order the clasification in CORINE legends, from a year to another, in type of vegetation?

For example, CORINE clasificate a zone of pasture one year, but in the next temporal clasification give crop of dry cereals, and preview clasification don't find. How can I order then?

20 October 2020 3,913 1 View

Does the molecular weight of PVP matter in CTAB buffer for DNA and RNA extraction?

I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...

05 September 2020 7,488 4 View

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Online data repositories are useful tools that ensure the reproducibility and repeatability of scientific investigations. For the sake of facilitating collaboration between researchers in the...

31 August 2020 4,826 3 View

best practices to check linear regression assumptions?

Hi everyone! I'm conducting a linear regression and I need to check the model assumptions. I wanted to ask what are the current best practices, and of you could provide a referente. Furthermore: I...

25 April 2020 7,888 17 View

Can the origin of the cognitive constructs that characterize us be produced by the coevolution of innate human cognitive capacities?

We know that the cognitive capacities that most characterize us are theoretically constructs, that is, non-observational concepts that do not have a concrete existence similar to the physical...

19 March 2020 2,427 3 View

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In general, football is a sport deportation that moves to a large market and related businesses, as well as dealing with public limited companies in football teams also with commercial societies...

23 January 2020 2,340 1 View

Unusual annealing temperature of primers in rt-PCR??

Hi everyone, It may not be a problem really but...I was very surprised because although in manuals and from my experience, I know that the Ta can differ up to 5 degrees or more from the Tm of the...

19 November 2019 8,770 3 View

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23 February 2021 4,014 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the minimum centrifugation RPM and minimum time for TiO2 nanoparticle synthesis from plant extract?

In my laboratory, I have an analog centrifuge (does not show real RPM value). Its' brochure indicates the range is 300-3000 RPM. The regulator is marked from 1 to 10. Is there any way to determine...

01 February 2021 2,061 4 View

Does anyone have experience with NCI H716 cell line?

Hello! I am working with NCI H716 cells and I am struggling a bit trying to "wash" the cells since they are producing significant debris. I have centrifugated and resuspended them in their media...

28 January 2021 6,421 1 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

How do I know if my E.coli cells are fully lysed from sonication ?

Hi everyone, I am doing protein expression via E.coli. I am required to lyse the e.coli cells via sonication to obtain cell lysate. I normally sonicate 1L cell pellet suspended in salt buffer at...

22 December 2020 9,675 7 View

How to thaw whole blood for ELISA?

Hi all, I have frozen (-20C) samples of whole blood from mice from a recent experiment. I need to find a way to thaw them so as to extract sera for an ELISA, while minimizing hemolysis and...

08 December 2020 1,004 3 View

Should I use higher centrifuge speed or my polymerization actually did not work?

One of my sample (NIPs-non-imprinted polymer) couldn't settling down after centrifugation (4000 rpm). Might be due to very fine particles. So what I did, I let the samples to evaporate under fume...

06 December 2020 3,344 3 View