In the article we’ve read, only samples of the healthy individuals are acquired and used. We are curious about the sample of unhealthy volunteers. Would there be any difference between the two samples, or the results will also be the same?
Good day, Everyone! I hope this message finds you well. I am a third-year college student from Rizal Technological University, and I am reaching out to request assistance with the validation of...
28 May 2024 7,822 1 View
Anthropometrically and conceptually, association does not equate causation. However, regarding anthropometric indicators (i.e., metrics of general and abdominal obesity), most of studies worldwide...
20 April 2024 8,779 6 View
The observed increase in lactate dehydrogenase (LD) activity in underfilled tubes can potentially be mitigated through specific sample handling and processing techniques. What are the proper...
11 April 2024 3,937 0 View
This question is based on the paper "Tenets of Specimen Management in Diagnostic Microbiology" by Rajeshwar Reddy Kasarla and Laxmi Pathak. The article stated that optimal specimen collection...
07 April 2024 3,949 3 View
Hello everyone, I am interested to know how I have to calculate the concentration of a specific analyte during the digestion steps (salivary, gastric, intestinal...). During the method, the...
04 April 2024 9,366 1 View
child and spouse abuse from the past 50years
21 March 2024 3,765 0 View
I am not a designer I am just trying to understand how the temper of some PH steels is chosen when manufacturing clamp adjusters or fasteners. Also, what is the effect on torque or clamping forces...
05 March 2024 9,985 2 View
I'm planning a project focused on studying osteocytes in the long bones of rats, requiring intact sections of both cortical and trabecular bone. To achieve this, I'm considering Kawamoto's tape...
23 January 2024 3,172 2 View
Can I upload to ResearchGate an English translation of my book or article which was originally published in another language?
21 December 2023 8,532 2 View
Hi! We found this plant in the cloud forest of central Veracruz, at around 1700 meters above sea level, in riparian vegetation. We believe it belongs to the Solandra genus; however, we haven't...
17 December 2023 9,134 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
isolation of microplastic from sludge sample using centrifugation ..
23 July 2024 6,418 0 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View
We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...
23 June 2024 4,279 0 View
Dear colleagues, does anyone know of a suitable alternative for Centrifuge software (calculating settling time for grain size separation by centrifugation), preferably one running on Windows 10 or...
17 June 2024 7,085 0 View
I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....
16 June 2024 4,381 0 View
I'm trying to synthesis a hydroscopic peptide using a microwave assisted solid phase peptide synthesiser. I'm using an already optimised cleavage process, the issue arises after precipitating the...
15 June 2024 2,728 2 View
Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...
09 June 2024 6,311 0 View