Is there any database containing info related to the several kinase mutants generated by the clinical use of kinase inhibitors?

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How can I measure antioxidant enzymes in "troublesome" seeds from a native tree?

Dear colleagues, I have been trying to get crude extracts for the determination of the activity of antioxidant enzymes in seeds of a native tree species (Bignoniaceae). Embryos were separated...

19 May 2024 7,422 0 View

How can I measure the activity of antioxidant enzymes in seed embryos of a native tree (Bignoniaceae)?

Dear colleagues, I have been trying to get crude extracts for the determination of the activity of antioxidant enzymes in seeds of a native tree species. Embryos were separated and ground with...

16 May 2024 4,683 0 View

How to change reference group between a three-group Cox hazard model for a survival meta-analysis?

Example of data extraction from a study during a meta-analysis: A: reference. B: HR (95%CI) C: HR (95%CI) I am running a meta-analysis that combines B vs A+C. I could combine groups A+C using an...

15 April 2024 1,478 0 View

Are artificial reefs from the dismantling of oil platforms a good strategy?

I'd like to know what you think about converting decommissioned platforms into artificial reefs. Is this one of the most viable options, both in economic and environmental terms? What are the...

07 April 2024 381 1 View

BED enhancer to tissue expression. How to?

Hi everyone! I have a list of genomic features in BED file format. They represent short features which are enhancer or silencer. I have to pick some for further investigation and I decided to...

24 September 2023 7,157 0 View

How can I resuspend homogeneously a bacterial pellet for CFU counting?

We are currently culturing several bacterial strains which grow as clumps in liquid medium (without agitation because they are micro-aerophillic) for CFU counting at a given OD (0.8 for instance),...

20 September 2023 8,758 1 View

Problems with Biotin binding on Streptavidin magnetic beads?

I saw some other people presenting similar problems. For a few years now, we were using Dynabeads M280, coupling them to a biotinylated antibody to perform immunopurification of target molecules....

23 July 2023 5,051 4 View

GAMMs for speech analysis?

Hello everyone, I'm looking for theoretical and experimental material on Generalized Additive Mixed Models (GAMMs), particularly for the purpose of speech analysis. If anyone could recommend...

17 July 2023 1,142 2 View

Is there any solver to perform electrodeposition in OpenFOAM?

Is there any OpenFoam opensource solver that solves navier-stokes, nernst-planck, butler volmer and mass transport equation? reference work by Kauffman et al 2020 Multi-Physics Modeling of...

20 June 2023 674 0 View

How can i obtain DDEC6 charges with a CASTEP calculation?

Hello to every one, I'm trying to test various types of population analysis on CASTEP calculated charge density. I would like to perform a population analysis with chargemole. I have obtained the...

11 June 2023 864 1 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View