At our lab, we frequently grow Staphylococcus aureus using TSB or Chemically Defined Media. The latter gives us some control on the conditions we want to impose to the organism, but I was interested in knowing if there is any media that mimics the conditions that the organism faces during human infection? (if there isn't, do you think there is any good approach to achieve this?)
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciz106/5308510
Hello, see info below may be useful for you.
Good luck!
https://www.google.com/amp/s/medicalxpress.com/news/2019-04-intradermal-staphylococcus-aureus-infections.amp
For the growth of Staphyloccus aureus Mannitol Salt Agar may be the good medium.
@ Filipe Medina de Sousa Here, I recommend Mannitol salt agar (MSA) for S. aureus. Because, Staphylococcus can ferment mannitol, an acidic byproduct is formed that causes the phenol red in the agar to turn yellow. Hence, it has used for the selective isolation of presumptive pathogenic Staphylococcus species. In addition, Congo red agar (CRA) also used to examine the potential of slime layer formation for their pathogenicity of S. aureus inside host cell.
Dear Filipe Medina de Sousa
there is artificial sputum medium that is used to research cystic fibrosis bacteria. Here is a link to one of the recipes:
https://protocolexchange.researchsquare.com/article/nprot-1999/v1
It is also an option to use a diluted clinical material e.g. sputum, blood/serum. However, you have to have access to patients.
My colleagues were using the second approach, for details see method section in the attached article.
Hope it is helpful
Kind regards
Jan
- Badges
- Science topic
- Similar topics
- Biological Science
- Microbiology