im working with the yeast strain BY4741, the double mutant (cta,ctt) that i have previously transformed with 4 diferent catalase T variants insterted in the plasmid pRS316, which confers the ability to grow in media without uracil, and I have transformant colonies of each variant but theres one that, despite the uracil selection, shows no apparent catalase activity
My advisor told me i could use paraquat or H2O2 to "force" the catalase to express, Im looking for media recipes and for the safety measures needed when manipulating paraquat, hope theres any...
See "Reactive Oxygen Species-mediated Regulation of Mitochondrial Biogenesis in the Yeast Saccharomyces cerevisiae*
Cyrille Chevtzoff,‡§ Edgar D. Yoboue,‡§ Anne Galinier,¶‖ Louis Casteilla,¶ Bertrand Daignan-Fornier,‡§ Michel Rigoulet,‡§ and Anne Devin‡§,1" No special precautions need be taken except addition of media sterialization.
Hello Ileana,
in the attachment you will find the safety data sheet for paraquat (methyl viologen). It is a toxic substance, so you should know this when working with it.
Regards, Christian
please read the methodology
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979731/
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioscience
- Biotechnology