Is there any correlation between gene expression and protein synthesis?

More Mahdi Yahyazadeh's questions See All

How pharmaceutical company separate medicinal alkaloids in big scale?

There are several standards of alkaloids in a company such as sigma with low price. using Chromatography system such as Preparative-HPLC can increase the price of final product. therefore, they...

05 June 2018 8,720 2 View

Does anyone know where can I download LC-MS libraries?

I am looking for characterization of some unknown components probably alkaloid that were extracted from Catharanthus roseus.

02 March 2018 9,430 0 View

What is the reproducibility of counter current chromatography (CCC)?

CCC is a preparative method, but I would like to know whether every run likes HPLC have the same retention time or not?

01 February 2018 9,396 1 View

Where can I find a full library of alkaloid analyzed by LC-MS?

I analyzed C. roseus plant extract by LC-MS. I found some unknown peak that I could not find any comparable fragmentation in literature for them. They should probably be alkaloids? How can I...

01 February 2018 2,518 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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08 August 2024 1,668 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

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05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Transfection in HEK293T cells?

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31 July 2024 9,892 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

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PCR showing no bands - Master mix and primers didn't mix?

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31 July 2024 2,404 6 View

Deniz Kom

It is not %100 accurate. From gene to protein there are some more steps.

epigenetic regulations (post transcriptional modifications) are widely increasing or decreasing mrna turning to protein.

Alex Young

As Deniz Kom says, there is not always a 1:1 ratio of mRNA to protein translation. This ratio varies from gene to gene.