Is there any correlation between gene expression and protein synthesis?

More Mahdi Yahyazadeh's questions See All

How pharmaceutical company separate medicinal alkaloids in big scale?

There are several standards of alkaloids in a company such as sigma with low price. using Chromatography system such as Preparative-HPLC can increase the price of final product. therefore, they...

05 June 2018 8,577 2 View

Does anyone know where can I download LC-MS libraries?

I am looking for characterization of some unknown components probably alkaloid that were extracted from Catharanthus roseus.

02 March 2018 9,303 0 View

What is the reproducibility of counter current chromatography (CCC)?

CCC is a preparative method, but I would like to know whether every run likes HPLC have the same retention time or not?

01 February 2018 9,275 1 View

Where can I find a full library of alkaloid analyzed by LC-MS?

I analyzed C. roseus plant extract by LC-MS. I found some unknown peak that I could not find any comparable fragmentation in literature for them. They should probably be alkaloids? How can I...

01 February 2018 2,400 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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28 February 2021 7,127 2 View

Deniz Kom

It is not %100 accurate. From gene to protein there are some more steps.

epigenetic regulations (post transcriptional modifications) are widely increasing or decreasing mrna turning to protein.

Alex Young

As Deniz Kom says, there is not always a 1:1 ratio of mRNA to protein translation. This ratio varies from gene to gene.