I am aware that I can buy singular enzymes, but I am wondering If I can buy a mixture of lysosomal components as one reagent.
Dear Saed,
The following link describes a kit which contains a mixture of lysosomes and can be purchased from Sigma Co.:
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/lysiso1bul.pdf
Hoping this will be helpful,
Rafik
Thank you Rafik for the answer.
However, this kit is used to isolate lysosomes from culture cells or tissues.
Hi Saed, lysosomal contents reflect cell functions and differ radically from cell to cell. We are talking about cell specific differences in ~30 hydrolases and many ROS generating enzymes.
02 March 2021 3,060 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?
23 February 2021 3,810 6 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
07 February 2021 2,006 1 View