Can any one let me know whether there is any commercial source of primary culture of human or rodent caudate nucleus primary culture
One of my scholars is about to complete a thesis on mindfulness, resilience, and wellbeing. This university requires thesis examiners from different parts of the globe. Should be an associate...
02 June 2024 860 1 View
Hi all, I have been working on invasion assay but i have encountered a problem recently. I have attached image below (where one of them showed clear morphology while another are dispersed? Your...
10 January 2024 6,994 0 View
Hi all, I will be working on NK92 and may I know if it is better to use retroviral or lentiviral vector if i would like to express a gene in the NK cell cell line? Thanks alot!
31 December 2023 3,276 1 View
How can i write DLL file in TRNSYS 17 after creating my component in proformas?
21 October 2023 3,659 0 View
I need how to model and develop mix proportion of HVFAC concrete and regressional analysis also.
30 August 2023 8,246 1 View
That is the weather data of the place is not in my TRNSYS weather library.
12 August 2023 4,865 0 View
My GelMA samples are not forming proper hydrogels. I used to obtain good hydrogels earlier and I did not change any components from my sample synthesis recipes. The only thing I doubt is that...
10 July 2023 9,144 2 View
How can current issues in both conventional and solid-state additive manufacturing processes? What are the various metallurgical solutions available for effectively eradicating interlayer defects...
11 April 2023 7,588 3 View
I'm trying to synthesize malonyl dichloride from malonic acid using POCl3 as the chlorinating agent. I'm adding dropwise the POCl3 at 0 Celsius degrees to the solid malonic acid and stirring the...
07 February 2023 7,447 1 View
We want to know the charge of a dipeptide (Mw < 1kD). Dipeptide is based on aspartic acid and its negatively charged. So, to prove that we have carried out 3% agarose gel experiment, tracking...
03 January 2023 5,649 10 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...
30 July 2024 8,707 1 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...
18 July 2024 7,024 1 View
Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...
10 July 2024 1,870 0 View
Using confocal imaging, I'm investigating the effect of a particular compound on mitochondrial membrane potential in live cells. I've stained the cells with varying concentrations of the compound...
29 June 2024 7,546 4 View
Hi, I am working with Invitrogen Seamless cloning which requires 200 ng/ul insert DNA fragment. Protocol recommends to use restriction enzyme to digest pre-cloned plasmid and then elute specific...
25 June 2024 971 2 View
Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?
29 May 2024 1,357 0 View