We know proteins degrade induced by many treatments or using various of regents. But can we degrade some specific proteins into fragments using very regular and simple organic acids? Is there any one who read some useful publications about this or exactly do this kind of work? I am very interested in the mechanism, for example, which amino acid is easily attacked by functional groups from the organic acids.
Your suggestion will be helpful.
Thank you.
I don't know if there is any (small & simple) acid that could degrade protein. But try look for Edman degradation. The reagent used is small, but perhaps not what you expected.
Radif
Thank you very much for your kind and helpful suggests, Muhammad Radifar and Steingrimur Stefansson!
Great, I will try formic acid and I have found some publications about it.
Thank you again.
Zhichao Lou
Hi Steingrimur Stefansson,
Do you have any literature talking about the mechanism that formic acid induce the protein degradation?
Thank you very much.
Zhichao Lou
this paper contains some good references to chemical cleavage
http://ac.els-cdn.com/S0014579313007230/1-s2.0-S0014579313007230-main.pdf?_tid=bd256834-c2ff-11e4-b3d3-00000aab0f6b&acdnat=1425536617_fa2e800812b9f391010eb4ca85e7538e
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