I wanted to test some sample against neuro-inflammation in-vivo. Is there any established animal model especially to test neuro-inflammation? If not available, is it possible to induce inflammation in brain with LPS?
Yes, it is possible to induce inflammation in brain with LPS (see attached paper). In addition, there are many animal models for meningitis out there for the study of blood brain barrier. All the best!
Article LPS-induced release of IL-6 from glia modulates production o...
Dear Nurul Adhwa,
Thank you so much for your paper suggestion. In this paper they collected the astrocytes and microglia and also maintained the co-culture system. But I am looking for any model/way where inflammation can be induced directly to the animal brain. if you have any idea can you share with me please?
Hello there. Most of the bacteremia-derived meningitis animal model involves intravenous tail vein injection with the bacteria, and not directly into the brain. This may be due to the very hard skull that covers the brain, and the fact that in reality, infection of the brain is initiated by either the invasion of bacteria in the blood or the CSF, and then migrates following the flow to the brain. The paper I provide you here explains the method on how the bacteremia-derived meningitis animal model can be made.
On the other hand, since you are thinking about doing an infection directly to the animal brain, maybe you can still proceed with that. Patients with brain trauma where their skull has been injured and fractured, pathogen can definitely enter directly into the brain in that sense. This is very interesting.
Article Interactions between Blood-Borne Streptococcus pneumoniae an...
Hello Dr. Nurul Adhwa,
I think this paper is quite impressive to produce meningitis and again take my cordial thanks.
Actually I am working with AD where neuroinflammation (due to activated microglia and astrocytes) plays a key role in neuronal loss. Hope I can try the same technique to produce such types of things.
I'm presuming you don't want to go with experimental autoimmune encephalomyelitis but if you do there are a number of mouse and rat models you can use.
I been looking for the same and found this article, hope might be helpful. Sadly there's no one main standardized model of neuroinflammation induced by LPS. While others are associated to Parkinson (6-OHDopa admon.), Alzheimer (transgenic strains) or Huntington (R6/1 or R6/2 strains).
Dear Guadalupe,
Thank you so much for attaching this article. It is really interesting and helpful.
Check the EAE mouse model. A good one to induce brain and spinal cord inflammatory injury similar to MS
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