Stripping buffer for fluorescent western?

Currently I am using from Thermo Scientific, however, it is unable to strip mouse Abs and very low potency in rabbit Abs. Do anyone have good experiences with commercially available stripping...

10 November 2017 6,636 6 View

What are the criteria to design primers for PCR?

31 December 2016 3,873 8 View

Is the molar absoptability of a compound is same in UV and fluorescence light?

Hello, I am wondering if the molar extinction co-efficient (molar absorptibility) of a compound is same in UV as well as in fluorescence light. If there is any differences then what the extent of...

08 September 2016 1,480 3 View

Can acetic acid induce inflammatory mediators in the blood serum?

11 December 2014 9,510 4 View

Is there any animal model available to test neuro-inflammation?

I wanted to test some sample against neuro-inflammation in-vivo. Is there any established animal model especially to test neuro-inflammation? If not available, is it possible to induce...

11 December 2014 1,944 9 View

How can I detect protein phosphorylation and hyper-phosphorylation (multiple site phosphorylation) ?

Specially, in case of tau phosphorylation, the single residue phosphorylation can be detected through western blotting. Is it possible to detect multiple site phosphorylation in one blot? or any...

11 December 2014 839 6 View

Can anyone suggest how to cryopreserve the SH-SY5Y neuroblastoma cells?

I tried with 50% FBS and 10% DMSO; 10% FBS and 5% DMSO; 5% FBS and 10% DMSO. But when recultured, in all the cases, around 30% of cells become dead.

10 November 2014 2,092 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View