Is there any alternative reagent for (Triton X-100) to prepare a positive control used in the Hemolysis assay?

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For how long can I store samples fixed by Glutaraldehyde before SEM imaging?

I want to fix biological cells on metal substrate using Glutaraldehyde, so is it possible to store the samples for SEM imaging? Would this affect the samples? And what is the max. period of time...

07 August 2018 8,845 8 View

Is there a relation between (Alpha and Beta phases) and (Anatase and Rutile phases)?

I'm making XRD analysis for annealed pure titanium and titanium managanese alloy and I could determine whether if the material is in anatase or rutile phase, but how can I determine if it's alpha...

06 July 2018 2,147 3 View

What is scientific reason behind using Sodium Hydroxide for mapping the grain boundaries in metals?

I need to map the grain boundaries of metal, and I want to understand why Sodium Hydroxide can be used for this?

06 July 2018 3,934 4 View

For Anatase titanium nanotubes, what does it mean if I get only four raman vibrational modes from the expected six modes of the anatase titania?

The four identified peaks at 154, 404, 522, 642 (cm-1), are these peaks enough to declare it as anatase titania?

06 July 2018 6,349 0 View

What useful data rather than of identification the crystalline phases, can I get from the XRD of metal oxide?

Can I get any other useful data?

11 December 2017 7,479 0 View

Is 1,1,1,3,3,3- hexafluoro-2-propanol supplied in a liquid form? why its size is described in grams by Sigma Aldrich and Alfa Aesar ?

Read 1 answer by scientists to the question asked by Alaa M. Soliman on May 8, 2016

04 May 2016 1,466 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

I need JCPDS file of LSFCO nanomaterial. Can anyone provide me?

Require for Rietveld refinement in XRD.

08 August 2024 3,081 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View