Dear Richa,

Al alternative for XTT assays, are CTB assays. It's also a metabolic dye, but the read-out  is fluorescence instead of absorbance. Metabolic dyes are easy to use for fast screenings. If you want to look at survival, you can consider plating assays (which is more time-consuming.

For all kind of assays on Candida albicans biofilms, you can have a look at publications from Karin Thevissen.

Good luck,

Tanne 

Thankyou so much Dear Tanne and Kunal for your suggestions, I will also try them as alternatives

You can as well use crystal violet (CV) staining but that quantifies the biofilm mass rather than measure the biofilm's metabolic activity. Which makes more sense because the cells with a strain's could metabolise XTT differently.

Good luck

You can as well use crystal violet (CV) staining but that quantifies the biofilm mass rather than measure the biofilm's metabolic activity. Which makes more sense because the cells within each strain could metabolise XTT differently.

Good luck