How can I find the effect of different parameters like (Reynolds number, Hartman number, volume friction etc.) in COMSOL?

Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.

23 February 2021 9,086 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

Can we use image data in MATLAB Neuro Fuzzy Designer ?

I have remotely sensed data (image) that I want to use in the Neuro-Fuzzy Designer apps of MATLAB. Is it possible to use an image file in the Neuro-Fuzzy Designer app or only numeric data is...

17 February 2021 1,205 5 View

Is it possible to determine % K2O of organic manures by STPB method ?

To determine % K2O of organic manures, flame photometric technique is used whereas in inrganic fertiliser for K2O determination STPB method is used. I am trying to find out any method of...

24 January 2021 4,714 3 View

How to tackle "CONSTRAINT CONTROLS" error while using tie between CFRP and frame segment in abaqus?

Hello, I had been trying to model a portal frame wrapped with CFRP in ABAQUS. To tie the CFRP laminates with frame I need to use slave/master surface interaction. But whenever I try to model the...

19 January 2021 2,906 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

Why the mixture of NaOH solution (10-12M) and Na2SIO3 solution is getting solidify after mixing?

These days I am noticing that the mixture of NaOH (NH) (10-12M) and N2SiO3 (NS) solution gets solidify after 24 hours. The ratio of mixture of NS to NH is 2. To mention, the same solutions that we...

18 January 2021 7,911 2 View

Which model is most suitable for urban simulation?

Respected sir/mam, I want to simulate the urban expansion using different time series LULC based on satellite image. please suggest me most suitable model for urban simulation. Thanks and regards.

17 January 2021 9,134 11 View

Is there any packages or code of MATLAB for solving a system of stochastic delay differential equation?

Hello, Can you help me regarding MATLAB solver for a system of stochastic delay differential equations? To be specific the system is delay differential equation where parametric stochasticity is...

15 January 2021 6,950 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

No title

22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View