Anindhya Sundar Das

4 Questions 9 Answers 0 Followers

Questions related from Anindhya Sundar Das

How can I separate ribosome-free cytosol using sucrose cushion?

I intend to separate ribosomal and non-ribosomal fractions of cytosol using 20% sucrose cushion. I have already run the cell lysate on top of the 20% sucrose solution for 2 hrs at 1,39,000 g. I...

26 June 2016 705 2 View

Is there any alternative buffer system to barbital buffer?

I need to check relative electrophoretic mobility between oxidized LDL and native LDL in agarose gel electrophoresis. The buffer system conventionally used in this electrophoretic system is...

06 August 2015 8,156 3 View

Why do my PMA treated THP1 cells have black dots?

I treat THP1 cells with 5 ng/ml PMA for 48 hrs to differentiate. Recently, after 24hrs of PMA treatment I see black dots in the cytoplasm. These PMA treated cells are not properly responding to...

06 May 2015 9,274 4 View

Why are my PMA differentiated THP1 cells not responding to LPS induction?

I have differentiated THP1 monocytes into macrophage using 5 ng/ml PMA for 48 hrs. Then after washing twice with DPBS i have kept the diffrerentiated cells for 3 hrs in serum free media. Then I...

29 March 2015 6,801 8 View