I am looking for ways to determine Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid (CAS 53188-07-1)) in tissue/body fluid samples. Is anybody aware of existing published assays? Trolox is often used as a reference compound for antioxidant activity itself, but methods for its quantitation are somewhat hard to find.
Hi Christof,
The only paper I have seen on this topic is in the Journal: Biomedical Chromatography, 2012 Oct;26(10):1228-33. doi: 10.1002/bmc.2683, Determination of lipoic acid, Trolox methyl ether and tocopherols in human plasma by liquid-chromatography and ion-trap tandem mass spectrometry. It is interesting to note that the article suggests that their are no other publications in this area and the article has not yet been cited to date.
Thank you John! Yes, I also find it odd that there is so little available on the compound, since it has relatively widespread use in clinical research. Let's wait awhile if there are other ideas.
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