I wanna check the ability of one bacteria to produce chitinase enzyme, in the literature it showed some methods to obtain the colloidal chitin, but last time I tried it, it failed, maybe because of the filtration step or some other reasons, if someone have experience with that, what could be an easy way for preparing colloidal chitin ?
We have prepared colloidal chitin by HCl acid wash and filtration through cheesecloth. The attached paper may be of help. They are from a Brazilian group who does this sort of thing regularly..
Thats so helpful, thanks so much for for help. excuse me for asking about some more setails in the protocol;
to weight the colloidal chitin after fileration and washing with water, dont we have to dry it first in oven so that we measure the dry weight? and to add it to the media, should we consider the dry weight ?
Thanks so much for helping Dr. Brigham and Dr. Ghosh..
it feels good to see people sharing their experiences eventhough I'm asking basic details but as you now some simple protocols are difficult id your doing it for the first time following a written manuscript.. Thanks again
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