Dear members,
Can anyone kindly suggest me about suitable in-vitro model to study ischemia. I tried with neuro-2a cell lines by inducing apoptosis by various concentration of cocl2. it induced apoptosis but i could not found up-regulation of any related gene (TNF-a, Fas). I want to make a in-vitro ischemia model to study Fas mediated apoptosis and want to check my developed fas targeting peptide. Kindly suggest me about cell line and developed protocol.
Regards
Did you try Oxygen-glucose deprivation (OGD ), which is widely used as an in vitro model for ischemia? In order to perform OGD, cell or tissue cultures are usually incubated in a glucose-free medium under a deoxygenated atmosphere.
Dear Irfan,
I agree with the above comments regarding OGD. Although not perfect, its a way of mimicking at least two parameters
Dear Akopian, I did not try for OGD this time, I am agree OGD can mimic ischemic condition but most of research uses primary cells while i want to use cell line. can you kingly suggest me preferable cell line to mimic brain ischemic environment. ????
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