Is there an assay kit available for analysing circulating cytokines of dogs?

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A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

Does it work as antibiotic resistance if plamid has no RBS??

I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...

11 July 2024 7,847 1 View

How to deal with lab test values above quantifiable limit?

Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...

22 June 2024 6,181 1 View

How can I adjust my fabrication method of liposome in order to correctly photographed TEM structures of liposomes?

I preprared curcumin-loaded liposome by Thin Film Hydration Method using lecithin, curcumin and beta-sterol. I stained it with phosphotungstic acid and uranium acetate and took TEM images of each....

30 May 2024 6,863 2 View

Does the percentage of damage between the controls (negative and positive) differ much?

My negative control cells had some damage, they are neoplastic cells and my anaplastic cell had even more damage in the negative control. Ok, but my question is, is there much difference between...

27 May 2024 7,529 2 View

Are there people who success to staining pax7 on mouse tissue (frozen, 4%PFA)?

I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...

27 May 2024 1,597 1 View

Any low-cost mid-infrared spectrometer?

If we build up a mid-infrared laser system and want to measure its spectra, where can I find the lowest-cost mid-infrared spectrometer, ranging from 2 to 20 μm。 Any recommendation? Thanks!

26 May 2024 8,114 1 View

What is the meaning of intersection point in transfer curve ?

Hi, I'm doing research n-type neuromorphic transistor device. According to theory and other research, forming EDL (electro double layer) can cause anti-clockwise transfer curve. However, as you...

26 May 2024 5,402 1 View

An error occurs when preparing ligands in Discovery Studio?

Hello, I am experiencing an error while using Discovery Studio and would like to ask for your assistance in identifying the cause. The details of the error are as follows: [ 22-May-2024 14:59:14...

21 May 2024 4,980 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

How can I find a subject for PhD?

I need help for find a subject for PhD in insect physiology

12 July 2024 8,227 1 View