Is there an antibody to the B7-H1 (PD-L1) molecule with very high sensitivity and specificity that works on paraffin-embedded sections?

03 November 2014 1 8K Report

Do you know or have an antibody to the B7-H1 (PD-L1) molecule that has a very high sensitivity and specificity that works on paraffin-embedded sections?

Jan Voskuil

EB06621 works in paraffin-embedded placenta, see http://everestbiotech.com/product/goat-anti-cd274-pd-l1-antibody/

Badges
Science topic

Similar topics
Mathematics
Algebra
Matrix

More Said Dermime's questions See All

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

My new vision on “time duality” in heat and wave equations. How can we catch their solutions and qualitative properties?

Greetings mathematicians and physicists, As we know, the idea of assuming a higher space dimension than the natural 3 dimension is effective in solving many physical and mathematical...

29 June 2024 9,220 6 View

How can I correct a mistake in the article? The title is wrong?

The definition of me appeared as the title of the article

07 June 2024 8,805 1 View

Are review articles/papers considered inferior and undervalued in academic research?

13 May 2024 251 3 View

The sum of Kinetic energy and Potential energy is called the Totalenergy Te=Ke+Pe, what can we say about the difference Ke-Pe and what can we call it?

Hello, The sum of Kinetic energy Ke(t) and Potential energy Pe(t) in a physical system is referred to as Total energy Te(t)=Ke(t)+Pe(t), what can we say about the difference Ke(t)-Pe(t) and what...

30 April 2024 5,928 6 View

How do I select the appropriate applied potentials for chronoamperometric to test the stability of electrode material in electrochemical HER/OER?

Currently, I’m working on electrochemical water splitting, specifically focusing on the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). I want to know how I choose...

18 March 2024 5,393 1 View

Literature Review for The Impact of Digital Transformation on Project Management ?

i need 6 previous studies containing The aim of this study, the type of the study,Results Recommendations, publisher, year of creation

17 March 2024 3,495 3 View

How to comment on flowcytometric results?

Tissue culture and flowcytometry

16 March 2024 5,198 0 View

Is it necessary to develop a questionnaire for conducting descriptive analysis before applying ARDL time series analysis to my data?

Is it necessary to develop a questionnaire for conducting descriptive analysis before applying ARDL time series analysis to my data

22 February 2024 576 1 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View