We have recently been using the RNAeasy plant RNA extraction kits from Qiagen. A supplementary kit allowing DNase treatment directly on the column is also available. This kit works out at £1 per reaction with the advantage of treating the RNA, to remove contaminating DNA, whilst it's still on the column prior to the elution step. Has anyone tried an alternate source of DNase to treat the column ?
Hey Andrew, good to see you here, long time. I dont use the RNAseasy kit, but I do have a good cheaper alternative in a paper by Oñate-Sánchez and Vicente-Carbajosa in 2008 BMC research notes ( http://www.biomedcentral.com/content/pdf/1756-0500-1-93.pdf ). For a protocol: https://maeda.botany.wisc.edu/wiki/Maeda_Lab:Protocols
Then it becomes super cheap, great Q RNA for qRT-PCR and whatever. The DNAse is the most expensive thing in this method, and I buy it from Fermentas/Thermo for about 50 bucks per 1000 Units.
I bet you can also use a cheaper DNAse on the QIAgen collumns though, worth a try.
Hope this works, cheers, Julian
I have used similar system from Macherei-Nagel. I can not recall the price/column of that though. Nevertheless nowadays we use another kit for DNase treatment which costs about twice as much per reaction. :)
Mol Biol is damn expensive. :(
I second the Fermentas/Thermo DNAse. In this part of the world is about 2 times as expensive, but still the most inexpensive on the market. I would also recommend to your attention MRCs RNAzol, which is improved Trizol. The protocol yields pure RNA without the need for DNAse, it can be used also for small RNAs and since it doesn't use columns it can be scaled up and down according the needs. And it is cost efficient.
Don’t waste a good Oiagen protocol (and your time/efforts) on incompatible reagents.
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