Is there a webtool to predict the outcome of amplification (suggesting the Tm) using primers with mismatches (not in the 3' end) in target sequence?

12 October 2015 1 7K Report

Do you know any webtool to predict the outcome of an amplification (and suggesting the Tm) using primers containing several mismatches (not in the 3' end) with the target sequence?

Franco Magurno

yes I tried but unsuccessfully because the setting part about mismaches is quite confusing me

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