Do you know any webtool to predict the outcome of an amplification (and suggesting the Tm) using primers containing several mismatches (not in the 3' end) with the target sequence?
yes I tried but unsuccessfully because the setting part about mismaches is quite confusing me
I would like to read about your experiences in the measurement of the color of liquid samples (wine, oil, or whatever) using a glass cuvette to calculate L* a* and b* parameters with the...
08 February 2021 6,619 4 View
Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...
11 January 2021 6,005 4 View
I am preparing microcapsules from complex coacervation procedure to encapsulate an oil phase. However, filtration to remove the aqueous phase results in a paste of aggregated particles. So I am...
25 October 2020 3,152 3 View
Looking for tools with proved accuracy to detect ploidy levels in microbial whole-genomes.
14 October 2020 4,273 3 View
26 July 2020 2,893 4 View
Dear colleagues, We all know that, ideally, human anatomy is better assessed in cadaver model. Artificial 3D-printed moldes, however, emerged to contribute to science. In order to develop...
02 June 2020 7,313 1 View
Dear colleagues, what is your approach for remote education during COVID-19 pandemic in terms of timing? What do you prefer? (1) Teaching live with classes pre-established at certain date (e.g....
21 May 2020 6,981 18 View
Hello, I'm using WRF-CHEM to simulate the Etna plume and fall-out. I'm using prep_chem_search.inp to input starting data, using Values.txt tu configure the height, duration, ash mass, and SO2 mass...
19 April 2020 5,503 1 View
I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...
26 March 2020 4,022 3 View
I just installed a new RTX 2080 on an old pc (I7 980x 12 CPU cores), downloaded and installed the latest nvidia drivers and CUDA toolkit for ubuntu, also I downloaded the namd build with CUDA...
30 July 2019 375 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View