Hi Naveen

You can use RNAlater from life technologies because of the following benefits.

Appropriate storage of cells or tissue samples prior to RNA isolation is critical for preservation of intact RNA within those samples. RNAlater is an aqueous solution designed specifically to maintain RNA integrity during storage of fresh tissue and cell samples.

For fresh tissue, simply cut samples to a maximum thickness of 0.5 cm in any one dimension and submerge in 5 volumes of RNAlater.

Cultured cells should be pelleted, resuspended in a small volume of PBS, then mixed with 5-10 volumes of RNAlater.

Once in RNAlater, samples can be stored for up to 1 day at 37°C, 1 week at 25°C, 1 month or more at 4°C, and long-term at -20°C or -80°.

Using samples that were stored in RNAlater is much easier than using frozen samples. Frozen samples must be ground to a powder and then the frozen powder must be transferred to a tube for homogenization. This procedure is laborious, messy, risks loss of sample, and perhaps most importantly, may lead to sample thawing, which can compromise RNA integrity. Samples stored in RNAlater are protected from RNases as long as the tissue remains in the solution, and they can typically be disrupted using the simpler methods appropriate for freshly collected samples (grinding in liquid nitrogen is only required for extremely hard or tough tissues such as bone or tumor tissue). Thus, in addition to making sample disruption easier, storage in RNAlater eliminates the risks of sample loss and mess due to transferring or thawing frozen powdered tissue.

RNAlater has been successfully tested with many different tissues, (including brain, heart, kidney, liver, skeletal muscle, fat, lung) and cell types (E. coli, Drosophila, cultured mammalian cells, and some plant cells). RNAlater can also be used to store anticoagulated whole blood or the white blood cell fraction of whole blood.

Tissue stored in RNAlater is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol®, and all of Ambion's RNA isolation kits. It is also possible to extract both genomic DNA and total protein from samples stored in RNAlater.

Samples in RNAlater can safely be shipped on wet ice for several days. For longer shipping times use dry ice

Best Regards

Hi Naveen

You can use RNAlater from life technologies because of the following benefits.

Appropriate storage of cells or tissue samples prior to RNA isolation is critical for preservation of intact RNA within those samples. RNAlater is an aqueous solution designed specifically to maintain RNA integrity during storage of fresh tissue and cell samples.

For fresh tissue, simply cut samples to a maximum thickness of 0.5 cm in any one dimension and submerge in 5 volumes of RNAlater.

Cultured cells should be pelleted, resuspended in a small volume of PBS, then mixed with 5-10 volumes of RNAlater.

Once in RNAlater, samples can be stored for up to 1 day at 37°C, 1 week at 25°C, 1 month or more at 4°C, and long-term at -20°C or -80°.

Using samples that were stored in RNAlater is much easier than using frozen samples. Frozen samples must be ground to a powder and then the frozen powder must be transferred to a tube for homogenization. This procedure is laborious, messy, risks loss of sample, and perhaps most importantly, may lead to sample thawing, which can compromise RNA integrity. Samples stored in RNAlater are protected from RNases as long as the tissue remains in the solution, and they can typically be disrupted using the simpler methods appropriate for freshly collected samples (grinding in liquid nitrogen is only required for extremely hard or tough tissues such as bone or tumor tissue). Thus, in addition to making sample disruption easier, storage in RNAlater eliminates the risks of sample loss and mess due to transferring or thawing frozen powdered tissue.

RNAlater has been successfully tested with many different tissues, (including brain, heart, kidney, liver, skeletal muscle, fat, lung) and cell types (E. coli, Drosophila, cultured mammalian cells, and some plant cells). RNAlater can also be used to store anticoagulated whole blood or the white blood cell fraction of whole blood.

Tissue stored in RNAlater is compatible with all commonly used RNA isolation methods, including single reagent isolation products like Trizol®, and all of Ambion's RNA isolation kits. It is also possible to extract both genomic DNA and total protein from samples stored in RNAlater.

Samples in RNAlater can safely be shipped on wet ice for several days. For longer shipping times use dry ice

Best Regards

Hi Naveen

You Can Also Use Preserved Blood RNA Purification Kit I from Norgen Biotek.

Its brochure is attached for your kind consideration.

Best regards

You can store your blood samples in Paxgene blood RNA tubes at room temperature for 7 days and at -20 degrees for longer periods.

following keenly

You can consider preserving your specimens in RNAlater as suggested by Sibtain Afzal