Is there a way to perform PCA on multifasta?

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PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Can holes for pressure probes in journal bearings modify the original pressure distribution?

It is very common to measure the pressure distribution inside the bearings with pressure probes connected to the oil film through holes in the bush. Once filled and pressurized, the probe lines...

12 June 2024 1,936 0 View

What should I do if non-specific bands appear only in specific target in western blot?

I tried western blot targeting TTF-1 (Thyroid transcription factor-1) also known as Nkx2.1. I tested two primary antibodies, one is Invitrogen, Nkx2.1 Monoclonal Antibody (8G7G3/1) and the other...

29 May 2024 3,517 3 View

Junichi nishizawas "failures"?

junichi nishizawa was a great scientiest and inventor of japan. I want to know about Junichi Nishizawa Failures and/or Criticism.

28 May 2024 4,547 1 View

How to fix " turbulent viscosity limited to viscosity ratio of 1.000000e+05 in xxx cells" ?

Hi everyone, I try to do a simulation about heat transfer of supercritical hydrogen in a cooling channel. Properties of H2 is obtained from NIST and displayed by an UDF function. I used the k-w...

16 May 2024 4,076 2 View

LPS doesn't work. What should I do?

I started to work with C8-D1A cells, mouse astrocyte cell line. We are trying to induce inflammation in cells using LPS, but treatment with LPS does not seem to induce inflammation. I also...

12 May 2024 608 3 View

How can be my research analysis plan?

How do I intend to integrate quantitative and qualitative data analysis techniques to comprehensively examine the intricacies of English and Arabic teaching and learning processes in second...

10 May 2024 1,493 5 View

Is crafting a challenging question for my Ph.D. thesis can be instrumental in driving scholarly discourse and advancing knowledge in my field?

In the context of global linguistic diversity and the increasing interconnectedness of societies, how can theories and practices in second language acquisition for English and Arabic be adapted...

10 May 2024 6,593 1 View

How can somebody help me to validate the methodologies?

What strategies did Ii utilize to validate the effectiveness and reliability of my chosen methodologies in investigating the processes of English and Arabic teaching and learning in second...

10 May 2024 4,111 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

David Eugene Booth

Probably not but R is very powerful, free, and does everything essentially. To get started download jarad Lander R for everyone available in the z-library. Good luck with your research Best wishes, David Booth

Teshita Uke Chikako

see this materials if related

Ette Etuk

Get minitab installed into your system. Enter the data. Click stat then multivariate then principal components analysis.