The mixture contains dsDNA which is degraded into strands between 10 and 200 bp in length. During degradation, priming regions might be damaged, so they have to be ligated after degradation.
Hi everyone! I currently have the issue that my proteins are all eluting at the salt wash that I apply during my IMAC (HisTrap 5ml HP column). My proteins are all His-SUMO-tagged. They are...
25 May 2024 8,144 3 View
By a 3D devil's staircase I mean the plot of the rotation number (or any invariant property distinguishing states of the system) as a function of the ratios w2:w1 and w3:w1, where w1, w2 and w3...
26 June 2023 357 0 View
Hello! I am curious about the minimum hybridization time for the custom locus-specific probes for FISH. I know that it should take at least 12 hours at 37 degrees Celsius, but is there an even...
31 May 2023 9,886 2 View
Hello, I am building a VAR modell in order to discover how (WTI) oil price shocks (changes in the WTI price) and 3 macroeconomic control variables (gdp_growth, Log-Interest rate, Log-exchange...
21 November 2022 1,157 19 View
XPS spectra of the Si2p core level usually show the spin-orbit splitting of this level (1/2 and 3/2). In the case of SiO_x, all spectra I have found show a particulary narrow component, which...
29 August 2022 3,305 4 View
Dear all, The last weeks I'm struggling with the thermal evaporation of aluminum. We're running the evaporator inside a nitrogen filled glovebox. I'm evaporating the aluminum with a pressure of...
13 April 2022 8,286 15 View
As I know, so far there has't been the attempt to combine those issues, but I'm not sure. Could be an interesting research item for urban studies
23 March 2022 2,671 6 View
Hi everybody! I am currently facing a very nasty protein that I am trying to purify. Besides the need for several purification steps, the lack of proper expression in insect cells gives me a hard...
02 February 2022 4,157 0 View
Dear Ladies and Gentlemen, I am currently working as a Master's student on several publications that methodically rely on latent profile analyses. In the context of these LPAs, I have repeatedly...
04 January 2022 1,194 4 View
For our manual on Romance languages in Africa (RoLA) at the University of Passau (Germany), we are looking for an expert on the variety of French spoken in Congo-Brazzaville who could contribute a...
08 December 2021 9,829 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View